PBS Buffer with Ca and Mg, pH 7.4, Sterile

PBS (Phosphate Buffered Saline) contains calcium and magnesium, unlike other forms that do not, and is at a pH of 7.4. Calcium and magnesium facilitate cell binding and clumping. A solution of PBS (1X) contains 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 1.8 mM KH₂PO₄. PBS is a water-based salt solution that helps maintain a constant pH. PBS (Phosphate Buffered Saline) is isotonic and non-toxic to most cells so therefore has many uses, including substance dilution and cell container rinsing.

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